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Protein & Nucleic Acid Interactions
Proteins are the workhorse molecule inside of a cell and are responsible for the myriad of biological activities that cells undergo to function and survive. Interestingly, a diverse set of proteins also interact with DNA. DNA within our chromosomes is often tightly bundled around proteins that are referred to as the chromatid within the chromosome. These protein-DNA bundles help package the DNA into a compact form within the cell nucleus. Powerful molecular tools and techniques have been developed by researchers to isolate these protein-DNA bundles and other DNA-binding protein complexes for further downstream applications.
Immunoprecipitation using highly specific antibodies to RNA and DNA-binding proteins (e.g. transcription factors), scientists can evaluate the regulation of molecular pathways and better understand gene function in both healthy and disease tissues. Numerous technologies and methods are currently available to investigate protein-RNA and protein-DNA interactions and are suitable for additional downstream analyses. For example,chromatin immunoprecipitation (ChIP) assaysare often used to investigate transcription factor-DNA interactions for gene expression and epigenetic modification studies. Whereas,RNA precipitation (RIP) assaysare commonly used to investigate proteins binding to mRNAs, non-coding RNAs, miRNAs, and viral RNAs. A common challenge with immunoprecipitation studies is the specificity and or access of the antibody to the protein of interest. To circumvent some of these issues, researchers will use recombinant protein technology to express modified proteins with unique tags, such as thehemagglutinin (HA) tag, that are recognized with high specificity by the appropriate antibody.Immunoprecipitation
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Interestingly, scientists leveraging protein-DNA technologies have devised novel tools and methods for evaluating protein-protein interactions. With high specificity and sensitivity,proximity ligation assay (PLA)technology facilitatesin situdetection of endogenous proteins, protein modifications, and protein interactions. Similar to immunoprecipitation techniques, the PLA assay uses highly specific primary antibodies to recognize the two proteins of interest. However, modified oligonucleotide-labeled secondary antibodies, functioning as the PLA probe, are used to bind to the primary antibodies. Only if both proteins are present and nearby will the hybridizing connector oligos join the PLA probes. The addition of ligase forms a closed, circular DNA template. With the newly formed circular DNA template formed, rolling-circle amplification is now possible with the addition DNA polymerase and ultimately generates a greatly amplified signal that is tethered to the PLA probe. The selection of the appropriate protein and nucleic acid interaction technology will largely be determined by the researcher’s downstream application needs.Proximity Ligation Assay Technology
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